A PROCESS FOR THE PREPARATION OF AN INSULIN DERIVATIVE

摘要

Human insulin is prepared by treatment of a porcine insulin product, preferably raw procine insulin, with carboxypeptidase A or a corresponding enzyme to form a desalanine-B30 insulin product, which is condensed, without the addition of buffer, for up to 30 minutes with a L-threonine alkyl ester in a mixture of a lower alkanol and water containing trypsin or an enzyme related thereto at a pH value of from about 5.5 to about 7.5 to form a insulin alkyl ester, which is hydrolyzed at a pH value of from about 8.5 to about 10.5 without adding enzyme and in the absence of NH4+ and HCO3- ions.