| 摘要 |
Mutant plasmids of Rts1 and P1 are provided which permit for high copy numbers of the plasmid in host bacteria. The mutant plasmids also possess other improved characteristics, including the ability to coexist with other vectors in host bacteria, such as E. coli, the ability to control copy numbers, within the range of about 20 to 120, by the use of different host bacteria growth medium, and the ability to remove the plasmids from host bacteria by a simple shift in the bacteria growth temperature. These plasmids may also contain an additional nucleotide sequence, GH3, which confers increased temperature stability. |
