Vaccines for suppressing IgE-mediated allergic disease and methods for using the same

摘要

Nucleic acid molecules that encode a protein comprising at least one epitope of membrane IgE free of epitopes present on the serum IgE, including proteins that further comprise non-IgE T cell helper epitope are disclosed. Vaccines, vectors and host cells that comprise such nucleic acid molecules are disclosed. Isolated proteins, including haptenized proteins, comprising at least one epitope of membrane IgE free of epitopes present on the serum IgE, including proteins that further comprise non-IgE T cell helper epitope are disclosed. Vaccines that comprise and methods of making such proteins and antibodies that specifically bind to such proteins are disclosed. Vaccines that comprise killed or inactivated cells or particles are disclosed. Methods of treating and preventing IgE mediated allergic disease or condition are disclosed.

主权项

1. A method of treating an individual who has been identified as being susceptible to an IgE mediated allergic disease or condition, the method comprising the step of administering to such an individual a prophylactically effective amount of a vaccine that comprises a nucleic acid molecule that encodes a fusion protein that comprises an epitope of membrane IgE and is free of epitopes of serum IgE, that encodes a non-IgE helper T cell epitope, and that encodes a proteolytic cleavage sequence between said epitope of membrane IgE and said non-IgE helper T cell epitope, wherein the proteolytic cleavage sequence is REKRAVYG (amino acids 140-147 of SEQ ID NO:6).