| 摘要 |
PURPOSE:To analyze easily the antigen and antibody in a sample by coupling a cell membrane which is subjected to cytolysis by complement activity to the antigen and antibody and providing a cell contg. internally a substrate reacting specifically with enzyme, and a substrate or enzyme reacting specifically with the enzyme or substrate in the cell thereto. CONSTITUTION:A titled reagent for immunological analysis is obtd. by coupling an antibody of which the antigen is a cell membrane to be subjected to cytolysis by complement activity to said cell membrane and mixing a buffer soln. contg. a cell for containing internally enzyme and a substrate. In analysis, a reagent, for example, the serum from a patient's blood is mixed with an antibody 2 capable of making antigen- antibody reaction with an antigen 1 which is the measuring object in the serum and complement 3, then the antigen-antibody reaction is caused by the antigen 1 and the antibody 2. When the liquid mixture subjected to the above-described reaction and the reagent are mixed, the remaining complement 3' is activated and a cell membrane 5 coupled to the antibody 7 is broken by the cytolysis thereof, by which internal enzyme 6 is released. Said enzyme 6 and the substrate 8 in the reagent react with each other and the resulting product 9 of reaction is obtd. Said product is quantitatively determined by a suitable means, whereby the antigen in the sample is analyzed. |
