| 摘要 |
<p>Compositions of fluorescent amplifier molecules are provided, as well as synthetic methods for their preparation. The procedures provide for the preparation of gram quantities of fluorescent molecules from available precursors. The fluorescent amplifier molecules include a multi-dimensional, rigid core (C), to which at least two fluorescent moieties are attached by way of a linker (L). Amplifier molecules that further include a 'targeting' molecule (a molecule that functions to direct the fluorescent amplifier to a particular binding site) are also presented, the targeting molecule being attached to the rigid, multi-dimensional core. Signal amplification without steric restrictions of fluorophore mobility is provided, hence reducing and/or avoiding interactions between moieties as well as fluorescence quenching. The advantage of the derivatives of the present invention, in addition to other things, over conventional fluorescent labels is due to the ability to amplify signal upon accumulation of the fluorescent moieties. The new amplified molecules are further disclosed to have the capability to quantitate the fluorescence signal. the compositions may also be employed as nucleic acid fluorescent -in-situ-hybridization probes.</p> |
