摘要 |
PURPOSE:To efficiently provide highly unsaturated fatty acids in a high concentration by hydrolyzing fats and oils with an enzyme having a 2-monoglyceride lipase activity and obtained by modifying Candida-cylindracea lipase with an alkali. CONSTITUTION:Candida-cylindracea lipase is treated with an alkali to modify the lipase into an enzyme having a 2-monoglyceride lipase activity. Fats and oils are subjected to a hydrolysis reaction using the modified enzyme under an alkaline condition of pH7.5-9 to specifically decomposed the ester bonds at the Sn-2 positions of triglycerides to provide highly unsaturated fatty acids. |