P300/CBP-ASSOCIATED TRANSCRIPTIONAL CO-FACTOR P/CAF AND USES THEREOF

摘要

The present invention provides a purified protein designated P/CAF having a molecular weight of about 93,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions and which acetylates histones and which also binds to the p300/CBP cellular protein. The present invention further provides a nucleic acid encoding the P/CAF protein as well as a vector containing the nucleic acid and a host for the vector. A purified antibody which specifically binds the P/CAF protein is also provided. Also provided are methods of screening for compounds that inhibit or stimulate the transcription modulating and histone acetyltransferase activity of P/CAF and p300/CBP.