发明名称 |
SYNTHESIS OF PROTEINS BY NATIVE CHEMICAL LIGATION. |
摘要 |
<p>Proteins of moderate size having native peptide backbones are produced by a method of native chemical ligation. Native chemical ligation employs a chemoselective reaction of two unprotected peptide segments to produce a transient thioester-linked intermediate. The transient thioester-linked intermediate then spontaneously undergoes a rearrangement to provide the full length ligation product having a native peptide bond at the ligation site. Full length ligation products are chemically identical to proteins produced by cell free synthesis. Full length ligation products may be refolded and/or oxidized, as allowed, to form native disulfide-containing protein molecules. The technique of native chemical ligation is employable for chemically synthesizing full length proteins.</p> |
申请公布号 |
MX9708500(A) |
申请公布日期 |
1998.02.28 |
申请号 |
MX19970008500 |
申请日期 |
1995.05.04 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
STEPHEN B. H. KENT;TOM W. MUIR;PHILIP E. DAWSON |
分类号 |
C07K1/02;C07K1/04;C07K1/08;C07K1/13;C07K17/06;C08G69/10;C08L89/00;(IPC1-7):C07K01/02;C07K01/04;C07K01/08 |
主分类号 |
C07K1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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