发明名称 |
Monacolin K biosynthesis genes |
摘要 |
Cholesterol inhibitor produced by Monascus, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.
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申请公布号 |
US7482157(B2) |
申请公布日期 |
2009.01.27 |
申请号 |
US20040022243 |
申请日期 |
2004.12.23 |
申请人 |
FOOD INDUSTRY RESEARCH & DEVELOPMENT INSTITUTE |
发明人 |
CHEN YI-PEI;LIAW LI-LING;WANG CHUN-LIN;LEE CHUNG-TSAI;HWANG ING-ER;TSENG CHING-PING;YUAN GWO-FANG |
分类号 |
C12N15/09;C12N15/16;C07H21/04;C07K14/39;C12N1/15;C12N1/16;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N9/88;C12N15/11;C12N15/14;C12N15/31;C12N15/52;C12N15/54;C12N15/63;C12N15/81;C12P7/62;C12P17/06;C12Q1/68;C12R1/645 |
主分类号 |
C12N15/09 |
代理机构 |
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