发明名称 |
ENZYMATIC MEMBRANE METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES |
摘要 |
The present invention provides a method for the enzymatic synthesis of peptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized reacting amino acids and uncharged or non-ionized peptide product in the presence of a proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the uncharged peptide product across an ion-rejection membrane which removes the uncharged peptide from the reaction mixture and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively "pulled" across the membrane. |
申请公布号 |
ZA8706115(B) |
申请公布日期 |
1988.04.27 |
申请号 |
ZA19870006115 |
申请日期 |
1987.08.18 |
申请人 |
THE COCA-COLA COMPANY |
发明人 |
GUILLERMO A. IACOBUCCI |
分类号 |
C07K1/00;C07K1/113;C07K1/14;C07K5/072;C07K5/075;C07K14/575;C07K14/655;C12P21/02 |
主分类号 |
C07K1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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