Method of detecting phosphatase

摘要

An easy operable method of detecting phosphatase which comprises a step for the production of a dye to obtain an azo dye by the reaction of phosphatase in a tissue or cell or on chromosome with a 2'-naphthol AS phosphate, specifically, 3-hydroxy- N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester, followed by the reaction between the dephosphorylated 2'-naphthol AS compound with a diazonium salt; an excitation step for the irradiation of excited light to the azo dye; and a detection step for the detection of fluorescence which is emitted upon irradiation of the excited light. The fluorescence is intense and lasts for a long time.