| 摘要 |
PURPOSE:To provide a new DNA fragment coding an esterase having high thermal stability and capable of forming an optically active carboxylic acid useful as a synthetic raw material for a physiologically active substance such as hypotensor. CONSTITUTION:A DNA fragment having a base sequence of formula II and coding an esterase catalyzing the asymmetric hydrolysis reaction of a carboxylic acid ester of formula I (R<1> is alkyl, aralkyl or aryl; R<2> and R<3> are independently alkyl; (n) is 1 or 2). It can be produced e.g. by cloning a chromosome of a microorganism capable of producing the desired esterase, e.g. Pseudomonas putida MR-2068 strain (FERM P-9677). |
