| 摘要 |
Processes for conjugating proteins with polyethylene glycol are disclosed. T he disclosed processes provide modified proteins having little or no decrease i n their activity and include the steps of deleting at least one amino acid residue on the protein, replacing the at least one amino acid residue with a n amino acid residue that does not react with polyethylene glycol, and contacting the protein with polyethylene glycol under conditions sufficient to conjugate the polyethylene glycol to the protein. This advantageous retentio n of a desired protein activity is attributed to the availability of one or mo re protein binding sites which is unaltered in the conjugation process and thus remains free to interact with a binding partner ligand or cognate subsequent to the conjugation process.
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