| 摘要 |
Discloses an immunohistochemistry (IHC) assay method for in vitro detection of oxMIF, wherein oxMIF is MIF which is differentially binding to antibody RAB4, RAB9 and/or RABO in a tissue sample of a subject, comprising a step of determining binding of a compound to oxMIF in said sample in vitro, wherein the following steps are carried out: a) blocking step with blocking buffer; b) binding step with primary anti-oxMIF antibody without a previous fixation step; c) fixation step; d) incubation with secondary antibody; and e) staining, wherein said compound binding to oxMIF is an antibody, specifically binding to oxMIF, wherein the antibody is an oxMIF binder selected from the group consisting of RAB4, RAB9, RABO, RAM4, RAM9 and RAMO. |
