INTENSIFICATION OF POLYMERISED CHROMOGENS IN IMMUNOCHEMICAL ANALYSIS

摘要

The intensification process in an immunoperoxidase analytical procedure, comprises marking an analytical sample with a peroxidase; contacting the marked sample with diaminobenzidine or similar chromogen so as to polymerise the latter as a precipitate on the sample; treating the sample with a water-soluble halogen complex of a Group VIII metal of Period 5 or 6 of the Periodic Table (such as a tetrachloroplatinate II); and further treating the sample with a physical silver developer containing dissolved Ag (I) so as to precipitate reduced silver where said diaminobenzidine has been polymerised and precipitated.