PROCESS FOR THE BIODEGRADATION OF AROMATIC COMPOUNDS AND SYNTHESIS OF PIGMENTS AND COLORANTS, ALCALOIDS AND POLYMERS, WITH THE USE OF THE RECOMBINANT STRAIN ESCHERICHIA COLI P260

摘要

The microbiological process for the biodegradation of aromatic compounds and synthesis of pigments and colourants, alkaloids and polymers, through the use of a recombinant strain E. coli P-260 is characterised by the constitutive expression of the enzyme 4-HPA hydroxylase of K. pneumoniae. This enzyme is involved in the degradation of 4-Hydroxyphenylacetic acid (4-HPA), one of the compounds present in black olive liquid ( @ alpechin @ ). The low specificity of the substrate of the enzyme and its double catalytic activity: A) Hydroxylation of monohydroxylated compounds into hydroxylated intermediaries and B) oxydation of di-hydroxylated compounds to quinones, provide for its double use: 1) degradation of toxic aromatic compounds of the ambient environment and 2) synthesis of organic compounds of industrial interest, of which the structure base is a quinone.