| 摘要 |
A new bilirubin oxidase enzyme and a conventional laccase enzyme oxidise bilirubin to biliverdin without the formation of hydrogen peroxide. The bilirubin conjugate in the biological fluid may be quantitatively determined by reacting it with bilirubin oxidase from a micro-organism derived from the genus Myrothecium, while the total bilirubin in the biological fluid may be quantitatively determined by the reaction of bilirubin oxidase or laccase, in the presence of a specific additive compound. In conventional enzymatic methods for the quantitative determination of glucose, cholesterol, neutral fats, free fatty acids, phospholipids or uric acid, all existing in the biological fluid, the interference in this determination by the bilirubin coexisting in the fluid can be prevented by adding bilirubin oxidase or laccase, with the specific additive compound, to the reaction system. <IMAGE>
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