Neues Verfahren zur quantitativen Bestimmung von extrazellularer DNS in einer biologischen Flüssigkeit

摘要

Process for quantitative determination of deoxyribonucleic acid (DNA) present in extracellular position in a medium. The process comprises the following stages: -the fixation of the DNA present in the medium, on a support, -the incorporation of at least one labelled deoxyribonucleotide into the fixed DNA, -the direct or indirect visualisation of the incorporated label. -Application to all situations in which it is advantageous to detect and/or quantitatively determine the DNA present in a medium in extracellular position such as, for example: -the situations in physiology or in pathology, in which it is advantageous to quantify in vivo or in vitro the intensity of the cell death phenomena, -in pharmacotoxicology, the in-vivo or in-vitro detection of the cytotoxicity of a toxic or medicinal substance, -the study of biocompatibility of the biomaterials employed in particular in extracorporeal circulation and haemodialysis systems, -the detection of DNA contaminating preparations of biologically active substances (in particular proteins or vaccines) obtained by genetic engineering techniques. a