HOMOLOGOUS RECOMBINATION IN MISMATCH REPAIR INACTIVATED EUKARYOTIC CELLS

摘要

The present invention relates to a method for modifying the genome of eukaryotic cells by homologous recombination using DNA sequences which substantially differ from the target locus with respect to the nucleotide sequence (0.1 to 30 % divergence) in the region where recombination takes place. Homologous recombination between diverged sequences is achieved by genetic or transitory inactivation of the cell's mismatch repair system.