SPECIFIC AND SENSITIVE METHOD FOR DETECTING NUCLEIC ACIDS

摘要

The invention relates to a method for detecting a nucleic acid comprising the production of a plurality of amplifications of a section of said nucleic acid with the assistance of two primers of which one can bond on a bonding sequence A of the nucleic acid and the other can bond on a bonding sequence C' which is complimentary to a sequence C. Sequence C does not overlap A and is situated in a 3' direction from A. The inventive method also includes bringing the amplifications in contact with a probe having a bonding sequence D which can bond on a sequence B, said sequence B being situated between sequences A and C, or the complement thereof. In addition, the invention relates to the detection of the construction of a hybrid out of the amplification and the probe, whereby the sequence situated between the bonding sequences A and C contains no nucleotides, said nucleotides not being linked to the bonding sequence D of the probe or to complement D' thereof.