PROCESS FOR PRODUCING VESICLE, VESICLE OBTAINED BY THE PROCESS, AND PROCESS FOR PRODUCING FROZEN PARTICLE FOR USE IN VESICLE PRODUCTION

摘要

[PROBLEMS] To provide a process for producing vesicles in which a desired substance can be encapsulated in a high proportion while regulating properties such as particle diameter to desired values. [MEANS FOR SOLVING PROBLEMS] A W/O emulsion is prepared from an aqueous solution in which a substance to be encapsulated is contained in a dissolved or suspended state and from an oil phase containing an emulsifying agent. Subsequently, the W/O emulsion is cooled to a temperature at which the aqueous solution therein becomes frozen particles and the oil phase retains its liquid state, and the oil phase is removed. Thereafter, an oil phase containing a vesicle-constituting lipid is added to the frozen particles, and the mixture is stirred to thereby replace the emulsifying agent present on the surface of the frozen particles with the vesicle-constituting lipid. An outer aqueous phase is added to the resultant frozen particles whose surface has been covered with a lipid film to hydrate the lipid film with the outer aqueous phase.