DNA MICROWELL DEVICE AND METHOD

摘要

An apparatus for carrying out a homogeneous nucleic acid amplification and nucleic acid assay on a liquid biological sample comprises a sample well (14) and an optical window element (20) which is received in the sample well (14). Opposed, spaced-apart surfaces of the optical window element (20) and sample well (14) define a capillary chamber (54) into which a liquid biological sample is drawn by capillary force. By spreading the liquid biological sample into a thin film within the capillary chamber (54) head space is eliminated, heat transfer to the sample is maximized, and a large optical target is achieved to facilitate the detection step of the assay. The disclosed apparatus is particularly suited for use with homogeneous nucleic acid amplification and fluorescence polarization assays, but can also be used in connection with other types of biological and chemical processes.