发明名称 |
A METHOD TO CLONE MRNAS AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET) |
摘要 |
The present invention relates to a two-step reverse transcription of DNA from RNA, wherein two types of reverse transcriptase enzymes are employed. The one type of reverse transcriptase enzyme having a temperature optimum at below 55 DEG C and the other type of reverse transcriptase enzyme having a temperature optimum above 55 DEG C, thereby obtaining cDNAs which are representatives of the entire gene, including the translated regions and at the same time performed effectively. <IMAGE> <IMAGE> |
申请公布号 |
WO9851789(A3) |
申请公布日期 |
1999.03.18 |
申请号 |
WO1998DK00186 |
申请日期 |
1998.05.13 |
申请人 |
DISPLAY SYSTEMS BIOTECH APS;WARTHOE, PETER, ROLF |
发明人 |
WARTHOE, PETER, ROLF |
分类号 |
C12N15/09;C12M1/00;C12N15/10;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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