| 摘要 |
Discloses a synthetic DNA sequence for expressing a protein of interest in maize cells which comprises: a) a codon-optimized DNA sequence encoding the protein of interest, b) at least one polyadenylation signal sequence chosen from the group consisting of Class I and Class II wherein; said codon-optimized DNA sequence contains at least one polyadenylation signal sequence from Class II and wherein said synthetic DNA sequence contains fewer Class II polyadenylation signal sequences than the protein’s native DNA sequence and contains the same number of class I polyadenylation signal sequences compared to said native DNA sequence. Also disclosed is a method of controlling pests by obtaining grain or seed from plants containing the claimed synthetic DNA sequences. |
