Targeted/immunomodulatory fusion proteins and methods for making same

摘要

Disclosed is a chimeric fusion protein for lysing of cancer cells comprising at least one targeting moiety to target a cancer cell and at least one immunomodulating moiety that counteracts immune tolerance, wherein the targeting moiety and the immunomodulating moiety are linked by an amino acid spacer of sufficient length of amino acid residues so that both moieties can successfully bind to their individual targets, wherein the immunomodulating moiety is TGF-&bgr;RII consisting of an amino acid sequence of SEQ ID NO: 4; wherein the amino acid spacer is selected from SEQ ID NO: 3 and SEQ ID NO: 11; and wherein the targeting moiety is an Anti-HER2/Neu antibody consisting of heavy chain SEQ ID NO: 1 and light chain SEQ ID NO: 2 or Anti-EGFR1 consisting of heavy chain SEQ ID NO: 5 and light chain SEQ ID NO: 6 wherein binding of the immunomodulating moiety TGF-&bgr;RII to TGF&bgr; inhibits proliferation of cancer cells.