Avian immunoglobulin-producing cell lines

摘要

The present invention relates to a method of preparing efficiently producing antibody avian cell clones. The method generally involves infecting a B-lymphocyte population with a v-rel gene using transfection or infection with a reticuloendotheliosis virus or reticuloendotheliosis virus and helper virus. The transformed B-cells are first isolated from the spleen or bursa, and then proliferated either in vivo in a second bird or in vitro in a liquid medium. Cell clones producing IgM, IgG or IgA may be selected. Prior immunization of the first bird with a desired antigen can be used to stimulate immunoglobulin producing cells.