发明名称 |
ELECTROPHORESIS METHOD AND APPARATUS FOR SEPARATING BIO-ORGANIC MOLECULES |
摘要 |
The present invention relates to the electrophoretic separation of bio-organic molecules using a slab gel electrophoresis apparatus having a pair of spaced, confronting plates defining a multi-lane separation zone adapted to hold a separation medium and an upper loading zone. In one aspect of the invention, at least one of the plates is shaped to provide an expanded loading zone. The plate-to-plate distance within the expanded loading zone is greater than the plate-to-plate width within the separation zone. In another aspect of the invention, a comb is provided with each tooth having a gradual taper beginning at a support member and extending along most of the tooth's longitudinal axis, and a sharp taper beginning immediately beyond the gradual taper and extending to the end of the booth. In a related aspect of the invention, a method is provided wherein a comb is inserted into the loading zone so that the teeth penetrate an upper portion of the separation medium, thereby forming a series of fluid-tight, elongate sample-loading apertures of substantially rectangular cross-section having a plate-to-plate width greater than that of the inter-plate distance in the separation zone.
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申请公布号 |
CA2288123(A1) |
申请公布日期 |
1999.10.17 |
申请号 |
CA19992288123 |
申请日期 |
1999.04.16 |
申请人 |
THE PERKIN-ELMER CORPORATION |
发明人 |
SELBY, BARTON G.;GOUDBERG, JOHANN;CLARK, DAVID;VO, BINH |
分类号 |
G01N27/447;(IPC1-7):B01D57/02 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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