Non-heating detection method for dermatophyte

摘要

The present invention provides a method of detecting dermatophyte, which does not require a complicated operation such as an enzyme treatment and a heat treatment.;The present invention provides a method of detecting dermatophyte, including a step of extracting a dermatophyte component from a sample with a treatment liquid containing a non-ionic surfactant or a zwitterionic surfactant, and a kit for diagnosing dermatophyte infection, containing a treatment liquid comprising the above surfactant, and an antibody specifically recognizing a dermatophyte component, which are housed in separate containers.

主权项

1. A method of detecting dermatophyte, comprising the following steps:
(a) a step of extracting a dermatophyte component from a sample selected from group consisting of skin, nail, scale, hair and pelage with a treatment liquid comprising 0.5-5.0 wt % of n-octyl-β-D-glucoside at a temperature of 4° C.-45° C., (b) a step of forming a complex by contacting the dermatophyte component obtained in said step (a) with an antibody produced by hybridoma 0014, and (c) a step of detecting the complex formed in said step (b).