Treatment of CNS disorders using descendants of MSCs expressing an exogenous Notch intracellular domain

摘要

There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which method comprises introducing a Notch gene and/or a Notch signaling related gene into the cells, wherein the finally obtained differentiated cells are the result of cell division of the bone marrow stromal cells into which the Notch gene and/or Notch signaling related gene have been introduced. The invention also provides a method of inducing further differentiation of the differentiation-induced neural cells to dopaminergic neurons or acetylcholinergic neurons. The invention yet further provides a treatment method for neurodegenerative and skeletal muscle degenerative diseases which employs neural precursor cells, neural cells or skeletal muscle cells produced by the method of the invention.

主权项

1. A method of treating a disease, disorder or condition of the central nervous system (CNS), the method comprising:
administering to a subject suffering from a disease, disorder or condition of the CNS a therapeutically effective amount of a population of neural precursor cells; wherein the population of neural precursor cells contains cells comprising exogenous nucleic acid sequences comprising Notch sequences; wherein said Notch sequences consist of sequences encoding a Notch intracellular domain; and wherein said administering results in a therapeutic effect on the disease, disorder or condition, thereby treating the subject.