发明名称 |
METHOD FOR SELECTING HYBRIDOMA PRODUCING ANTIBODY SPECIFIC TO NON-CONTACT CELL SURFACE ANTIGEN |
摘要 |
<p>The invention provdes a method for selecting hybridomas which produce antibodies specific to domains of a cell surface antigen which is usually not accessible at the surface of intact cells. The method employs the screening of the hybridoma clones obtained for the production of antibodies specific against the cell surface antigen by use of immunoblotting analysis, namely by screening the clones against antigen immobilized on a solid substrate such as nitrocellulose. The invention also includes those hybrodomas and monoclonal antibodies when produced according to the method.</p> |
申请公布号 |
JPS6229969(A) |
申请公布日期 |
1987.02.07 |
申请号 |
JP19860016959 |
申请日期 |
1986.01.30 |
申请人 |
ONTARIO KIYANSAA INST:THE |
发明人 |
BUIKUTAA RINGU;NOOBAATO KAATONAA |
分类号 |
G01N33/53;A61K39/395;C07K14/005;C07K14/195;C07K14/705;C07K16/00;C07K16/28;C07K16/44;C12N5/00;C12N5/10;C12N5/12;C12N15/00;C12N15/02;C12P21/00;C12P21/08;C12Q1/00;C12R1/91;G01N33/577 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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