发明名称 |
Method for rapid base sequencing in DNA and RNA with two base labeling |
摘要 |
Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
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申请公布号 |
US5405747(A) |
申请公布日期 |
1995.04.11 |
申请号 |
US19940208506 |
申请日期 |
1994.03.07 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA OFFICE OF TECHNOLOGY TRANSFER |
发明人 |
JETT, JAMES H.;KELLER, RICHARD A.;MARTIN, JOHN C.;POSNER, RICHARD G.;MARRONE, BABETTA L.;HAMMOND, MARK L.;SIMPSON, DANIEL J. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;G01N33/48 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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