| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide polypeptides useful for gene therapy purposes. <P>SOLUTION: A method for constructing a recombinant BAV vector containing a heterologous nucleotide sequence inserted into an insertion site, the method comprising: (a) linking the heterologous nucleotide sequence to sequences which have at least 90% identity to a BAV genome sequence comprising a specific sequence surrounding the insertion site, to form an insertion cassette; wherein the insertion site is located in E3 region; (b) introducing the insertion cassette into a cell, along with a second polynucleotide comprising a sequence having at least 90% identity to a BAV genome comprising a specific nucleotide sequence, wherein whole or a part of the E3 region is deleted; and (c) allowing homologous recombination to occur between the insertion cassette and the polynucleotide to generate the recombinant BAV vector. <P>COPYRIGHT: (C)2007,JPO&INPIT |
