GENE EXPRESSION TECHNIQUE

摘要

GENE EXPRESSION TECHNIQUE The present invention provides a method for producing heterologous protein comprising: (a) providing a host cell comprising a 2pm-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (b) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (c) purifying the thus expressed heterologous protein From the cultured host cell or the culture medium. FIGURE 16