| 摘要 |
<p>In a specific binding partner-mediated assay for an analyte, e.g. with a single binding site, a sample flows along a channel or permeable material from an application site 1. A labelled specific binding partner directed against the binding site on the analyte of interest is introduced in excess. At a site 2, a defined distance along the membrane or channel, there is immobilised a sufficient excess of the analyte to capture all of the labelled partner which has not previously bound the analyte of interest. The excess labelled partner thereby becomes immobilised, leaving labelled partner-analyte complex to continue to migrate along the material. At a further defined distance along the membrane or channel, i.e. at a 'test site' 3, there is immobilised a second binding reagent with high affinity for the labelled partner-analyte conjugate, upon which this conjugate becomes immobilised and can be determined.</p> |
