Use of non-<i>agrobacterium </i>bacterial species for plant transformation

摘要

The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

主权项

1. A method for transforming a cotton plant cell, comprising:
(a) contacting at least a first cotton plant cell with a Rhizobiales bacterium other than Agrobacterium sp. comprising: (i) a first nucleic acid comprising a vir gene region of a Ti plasmid wherein the vir gene region acts to introduce a nucleic acid of interest into the plant cell in a VirD2-dependent manner; and (ii) a second nucleic acid comprising one or more T-DNA border sequence(s) operably linked to a nucleic acid of interest; and (b) selecting at least a first cotton plant cell transformed with the nucleic acid of interest.