Accelerated directed evolution of microbial consortia for the development of desirable plant phenotypic traits

摘要

The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants.

主权项

1. A plant-mediated method for directed evolution of a plant microbiome, to create a microbial community associated with imparting a beneficial phenotypic trait to a plant, comprising:
a) growing a plurality of plants in a growth medium, in the presence of a first microbial community; b) selecting at least one plant from said plurality, following step a), based upon a beneficial phenotypic trait exhibited by the plant relative to other plants of said plurality; c) acquiring a second microbial community from the at least one plant selected in step b); d) repeating steps a) to c) in an iterative manner, wherein the second microbial community acquired in step c) is used as an inoculum in step a) of any successive repeat; and e) acquiring a microbial community that is associated with imparting a beneficial phenotypic trait to a plant.