Purification of herpes virus

摘要

The present disclosure provides a method to prepare purified enveloped viral particle preparations employing ion exchange chromatography and tangential flow filtration.

主权项

1. A method for the purification of herpes simplex virus (HSV) particles from a mammalian host cell culture comprising the steps of:
a) treating the mammalian host cell culture with a viral releasing agent to release the HSV particles from the host cells; b) treating the material from step (a) with an endonuclease; c) filtering the material from step (b) to remove any intact cells or cellular debris; d) applying the solution obtained from step (c) to an anion exchange chromatography resin; e) eluting the HSV particles from the anion exchange column; f) subjecting the eluent from step (e) to tangential flow filtration; and g) recovering the purified HSV particles.