System and method for sorting cells

摘要

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

主权项

1. A method of producing at least one sexed embryo comprising:
producing a stream containing sperm cells, wherein the stream comprises sperm cells from a cell source surrounded by sheath fluid, wherein the sheath fluid surrounding the sperm cells includes an amount of citrate; identifying X-chromosome bearing sperm cells and/or Y-chromosome bearing sperm cells in the stream; collecting X-chromosome bearing sperm cells and/or Y-chromosome bearing sperm cells in at least one collector having a collector fluid; and fertilizing at least one egg with the collected sperm to form at least one sexed embryo.