Plasma irradiation device for substance introduction and substance introduction method using plasma irradiation device

摘要

Disclosed is a liquid culture medium for substance introduction, which is capable of increasing the survival rate of cells after substance introduction as much as possible when the cells are irradiated with plasma for the purpose of introducing a target substance into each of the cells. Specifically disclosed is a liquid culture medium for substance introduction, which is used for the purpose of introducing a predetermined target substance into a cell and enables introduction of the target substance into the cell by having the cell in the liquid culture medium, which contains the target substance, irradiated with a plasma jet. The liquid culture medium contains a damage preventing component that prevents the cell from damage due to the plasma jet.

主权项

1. A plasma irradiation device for substance introduction for introducing a predetermined target substance into a cell by irradiating the cell in a culture container having a liquid culture medium containing said predetermined target substance with a plasma jet, wherein:
said plasma irradiation device comprises a stage section for supporting said culture container; a microscope section for observing cells in said culture container; a plasma generation section for generating said plasma jet; and a micromanipulator section for supporting said plasma generation section movably in a three-dimensional direction; wherein said microscope section further comprising a mirror and a predetermined object lens, wherein the mirror is downstream of the predetermined object lens, and the mirror is configured such that ultraviolet rays of the light incoming from the predetermined object lens are not led to an ocular optic part and said plasma generation section comprises a tube member composed of a tubular dielectric with its tip portion tapered off to a point and configured to allow a gas stream to flow therethrough, wherein said tube member is provided with a suction tube in a double tube style at its outside so as to enable a suction of said gas stream diffusing in an excessive amount from the opening at the tip portion of said tube member by means of a suction device connected to said suction tube, thereby preventing the cells from being damaged by the gas stream emitted from said opening at the tip portion thereof; and a double pole electrode disposed on an outer peripheral portion of said tube member, wherein said plasma generation section is configured such that, while flowing a gas stream containing a noble gas as a major component with oxygen mixed therewith through inside of said tube member, and sending a low-frequency high-voltage to each of the electrodes, an electric discharge is caused within the inside of said tube member and emits a plasma jet at a non-thermal equilibrium from an opening at the other end of said tube member, and is further configured such that said gas stream diffusing in an excessive amount from the opening at the tip of said tube member is sucked by a suction device connected to a suction tube part, whereby said predetermined target substance of interest may be introduced into the cell at a pin point by observing the cell by said microscope section while preventing the liquid culture medium from being infected with microorganisms by the production of free radicals upon introducing said target substance into said cell.