Chemical approaches for generation of induced pluripotent stem cells

摘要

The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.

主权项

1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising
(a) introducing into the mammalian non-pluripotent cell, one or more expression cassettes comprising an Oct4 polynucleotide, a Sox2 polynucleotide, and a Klf4 polynucleotide, and (b) contacting the cell of step (a) with a TGFβ receptor/ALK5 inhibitor and a GSK-3 inhibitor, thereby producing an induced pluripotent stem cell from the mammalian non-pluripotent cell.