| 发明名称 |
METHOD FOR REMOVING GENOMICALLY UNSTABLE IPS CELLS AND SYNTHETIC PEPTIDE USED THEREFOR |
| 摘要 |
The present invention provides a method that allows highly efficient and highly reliable evaluation of genomic stability of pluripotent stem cells, a method for removing pluripotent stem cells that have been identified as genomically unstable by the evaluation method from a culture of pluripotent stem cells to be evaluated, and a synthetic peptide that can be used for the methods. The methods provided by the present invention include preparing a culture of pluripotent stem cells of interest and analyzing an expression level of calreticulin for the pluripotent stem cells in the culture followed by identifying genomic stability or genomic instability of the stem cells on the basis of the expression level of calreticulin. |
| 申请公布号 |
US2016252523(A1) |
申请公布日期 |
2016.09.01 |
| 申请号 |
US201415033452 |
申请日期 |
2014.10.30 |
| 申请人 |
TOAGOSEI CO., LTD. ;KYOTO UNIVERSITY |
发明人 |
YOSHIDA Tetsuhiko;BAILEYKOBAYASHI Nahoko;YOSHIDA Yoshinori;CHONABAYASHI Kazuhisa |
| 分类号 |
G01N33/68;G01N15/14;C12Q1/68;C07K7/06;C07K14/47 |
主分类号 |
G01N33/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for evaluating genomic stability of pluripotent stem cells of interest derived from humans or a mammal other than humans, comprising:
preparing a culture of the pluripotent stem cells; and analyzing an expression level of calreticulin for the pluripotent stem cells in the culture followed by identifying genomic stability or instability of the pluripotent stem cells on the basis of the expression level of calreticulin, wherein in the identification, pluripotent stem cells, for which the expression level of calreticulin of the stem cells is above a prescribed level, are identified as genomically unstable. |
| 地址 |
Tokyo JP |
