摘要 |
Process and reagent for the specific determination of pancreatic .alpha.-amylase The present invention provides a process for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids, especially in serum plasma, duodenal juices or urine, by reaction with a system for the detection of .alpha.-amylase in the presence of a monoclonal antibody which reacts with salivary .alpha.-amylase, wherein a monoclonal antibody is used which specially inhibits the salivary enzyme but does not inhibit the pancreatic enzyme by more than 50%. The present invention also provides a reagent for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids, especially in serum, plasma, duodenal juices or urine, comprising a system for the detection of .alpha.-amylase and a monoclonal antibody against salivary .alpha.-amylase, wherein it contains a monoclonal antibody which specifically inhibits the salivary enzyme but does not inhibit the pancreatic enzyme by more than 50%. |