AMINO ACID MODIFIED POLYPEPTIDES

摘要

Incorporation of certain amino acid analogs into polypeptides produced by
cells which do not ordinarily provide polypeptides containing such amino acid
analogs is
accomplished by subjecting the cells to growth media containing such amino
acid
analogs. The degree of incorporation can be regulated by adjusting the
concentration of
amino acid analogs in the media and/or by adjusting osmolality of the media.
Such
incorporation allows the chemical and physical characteristics of polypeptides
to be
altered and studied. In addition, nucleic acid and corresponding proteins
including a
domain from a physiologically active peptide and a domain from an
extracellular matrix
protein which is capable of providing a self-aggregate are provided. Human
extracellular
matrix proteins capable of providing a self-aggregate collagen are provided
which are
produced by prokaryotic cells. Preferred codon usage is employed to produce
extracellular matrix proteins in prokaryotics.