发明名称 |
METHODS OF GENERATING FUNCTIONAL HUMAN TISSUE |
摘要 |
Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure. |
申请公布号 |
WO2016141137(A1) |
申请公布日期 |
2016.09.09 |
申请号 |
WO2016US20601 |
申请日期 |
2016.03.03 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
LEWIS, Jennifer, A.;SKYLAR-SCOTT, Mark, A.;KOLESKY, David, B.;HOMAN, Kimberly, A.;NG, Alex, H.M.;CHURCH, George, M. |
分类号 |
A61K35/545;A61L27/38;C12N5/071;C12N5/073;G01N33/15 |
主分类号 |
A61K35/545 |
代理机构 |
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代理人 |
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地址 |
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