Compositions and methods for using cathepsin E cleavable substrates

摘要

Provided herein are compositions and kits comprising a therapeutic agent linked to a cleavable substrate, wherein the cleavable substrate is capable of being cleaved by cathepsin E. Also provided are methods of treating one or more symptoms of a disease or disorder characterized by expression of cathepsin E in a subject and methods of eliminating a cancer cell characterized by expression of cathepsin E using the provided compositions and kits. Further provided herein are methods of detecting the presence of a cancer cell or detecting a cathepsin E expressing cell using a photosensitizer linked to a cleavable substrate, wherein the cleavable substrate is capable of being cleaved by cathepsin E.

主权项

1. A composition comprising a photosensitizer linked to a cleavable substrate, wherein the cleavable substrate is capable of being cleaved by cathepsin E and wherein the cleavable substrate consists of Arg-Gln-Ala-Gly-Phe-Ser-Leu (SEQ ID NO: 1), Gln-Ala-Gly-Phe-Ser-Leu (SEQ ID NO:2), Ala-Gly-Phe-Ser-Leu (SEQ ID NO:3), Gly-Phe-Ser-Leu (SEQ ID NO:4), Phe-Ser-Leu (SEQ ID NO:5), Ser-Leu (SEQ ID NO:6), Arg-Gln-Ala-Gly-Phe-Ser-Phe (SEQ ID NO:7), Gln-Ala-Gly-Phe-Ser-Phe (SEQ ID NO:8), Ala-Gly-Phe-Ser-Phe (SEQ ID NO:9), Gly-Phe-Ser-Phe (SEQ ID NO: 10), Phe-Ser-Phe (SEQ ID NO: 11), Ser-Phe (SEQ ID NO: 12), Ala-Gln-Ala-Gly-Phe-Ser-Leu-Pro-Ala (SEQ ID NO: 13), Gln-Ala-Gly-Phe-Ser-Leu-Pro-Ala (SEQ ID NO: 14), Ala-Gly-Phe-Ser-Leu-Pro-Ala (SEQ ID NO: 15), Gly-Phe-Ser-Leu-Pro-Ala (SEQ ID NO: 16), Phe-Ser-Leu-Pro-Ala (SEQ ID NO: 17), Ser-Leu-Pro-Ala (SEQ ID NO: 18), or Ser-Leu-Pro (SEQ ID NO: 19).