PROCESS FOR PREPARING RHESUS ANTIGEN, MEDICINE AND PHARMACEUTICAL COMPOSITIONS CONTAINING THE ANTIGEN AND ADMINISTRATION FORMS

摘要

1. A process for the preparation of Rhesus antigen for oral administration, from red corpuscles of the O Rh positive group not usable in transfusion therapeutics, characterized by the following steps : a) the red corpuscles separated from plasma and the white corpuscles are washed with a solution containing a phosphate buffer, pH 7.4 and glucose ; b) after removal from the washing solution, the red corpuscles are suspended again in a solution of CaCl2 , 0.025 M, in de-ionized water ; after about 1 hour, a phospho-calcium stroma precipitate is obtained which is separated by centrifugation ; c) the phospho-calcium stroma is washed with the washing solution according to a) diluted with de-ionized water and washing is repeated several times until a whitish solution is obtained which is lyophilized.