发明名称 |
PROTEIN GLYCOSYLATION MODIFICATION IN PICHIA PASTORIS |
摘要 |
<p>The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for ±-1,2-mannosidase I, glucosidase II, G1cNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the contemplated vectors are also included in this invention.</p> |
申请公布号 |
EP1516048(A2) |
申请公布日期 |
2005.03.23 |
申请号 |
EP20030735682 |
申请日期 |
2003.06.25 |
申请人 |
FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY (VIB) |
发明人 |
CONTRERAS, ROLAND;CALLEWAERT, NICO, L., M.;GEYSENS, STEVEN, C., J.;KAIGORODOV, VLADIMIR;WOUTER, VERVECKEN |
分类号 |
C12N9/12;C12N9/24;C12N15/81;(IPC1-7):C12N9/24;C12N9/10;C12P21/00 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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