NEW PHYSIOLOGICALLY ACTIVE SUBSTANCE, THEIR MANUFACTURE AND USE

摘要

PROBLEM TO BE SOLVED: To obtain a new physiologically active substance which is a ligand to an orphan G protein conjugate type receptor APJ expressing in a central nervous system, circulatory system, immune system or the like useful for the central nervous system function modifier, circulatory function modifier, immunological function modifier or the like. SOLUTION: This substance is a polypeptide having binding capacity to a receptor protein including same or practically same amino acid sequence to the amino acid sequence of the formula, it's precursor, their ester or salt. The subject polypeptide is, for example, is obtained in the processes, for example, purifying polypeptide from human tissue or cells, synthesizing the polypeptide in a known method, or culturing a transformant including a DNA coding for the polypeptide. The polypeptide is usable for quantitative analysis of APJ that is a G protein conjugating type receptor, development of receptor bonding assey system using recombination type receptor protein expressing system and screening of medicinae candidate compounds or the like.