| 发明名称 |
METHOD OF OBTAINING TERMINALLY DIFFERENTIATED NEURONAL LINEAGES AND USES THEREOF |
| 摘要 |
Provided is a method of inducing transdifferentiation of mesenchymal stem cells (MSC), the method including (a) culturing MSC in a first culture medium including a growth factor selected for allowing formation of neuralized MSC (NMSC); (b) allowing the NMSC to proliferate for a sufficient time during which said culture medium is renewed at least once; and (c) culturing the NMSC of (b) in a second culture media including cerebrospinal fluid (CSF) for a time sufficient for the NMSC to differentiate into a population of cells including terminally differentiated neurons, astrocytes and oligodendrocytes. Also provided by the present invention is the use of MSC or NMSC for providing a composition including said population and to kits including MSC or NMSC and instructions for use of same. |
| 申请公布号 |
US2016251619(A1) |
申请公布日期 |
2016.09.01 |
| 申请号 |
US201415029127 |
申请日期 |
2014.10.07 |
| 申请人 |
HADASIT MEDICAL RESEARCH SERVICES & DEVELOPMENT LIMITED |
发明人 |
KARUSSIS Dimitrios;KASSIS Ibrahim |
| 分类号 |
C12N5/079;A61K35/28;C12N5/0793 |
主分类号 |
C12N5/079 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of inducing transdifferentiation of mesenchymal stem cells (MSC), the method including:
a. culturing MSC in a first culture medium including a growth factor selected for allowing formation of neuralized MSC (NMSC); b. allowing the NMSC to proliferate for a sufficient time during which said culture medium is renewed at least once; c. culturing the NMSC of (b) in a second culture media including cerebrospinal fluid (CSF) for a time sufficient for the NMSC to differentiate into a population of cells including terminally differentiated neurons, astrocytes and oligodendrocytes. |
| 地址 |
Jerusalem IL |
