摘要 |
<p>A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or mor e in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressi ng a recombinant albumin coding sequence, wherein the cell has a reduced capaci ty of mannosylation of the recombinantly-expressed albumin.</p> |
申请人 |
DELTA BIOTECHNOLOGY LIMITED |
发明人 |
VAN URK, HENDRIK;SLEEP, DARRELL;CARTWRIGHT, ANDREW JOHN;WOODROW, JOHN, RODNEY;BEREZENKO, STEPHEN;CAMERON, JASON;GRANDGEORGE, MICHEL GASTON JOSEPH;MORTON, PHILIP HARVEY;MEAD, DAVID JOHN;BALLANCE, DAVID JAMES;VERON, JEAN-LUC BERNARD |